2009~2014    D-BSSE, ETH Zurich, Switzerland, Ph.D.

2006~2008    School of Electronic Science & Engineering, Southeast University, China, M.Sc.

2002~2006    School of Electronic Science & Engineering, Southeast University, China, B.Sc.

2014~present    School of Electronic Science & Engineering, School of Microelectronics, Key Laboratory of MEMS of Ministry of Education, Southeast University, China, Assistant Professor, Associate Professor, Professor

2014~2014        D-BSSE, ETH Zurich, Switzerland, Postdoc Researcher

Based on electronics and microelectronics, our interdisciplinary research focuses on the integration of microfluidics, biosensors, MEMS, embedded systems and AI into Lab-on-a-Chip systems applied in life science and precision medicine. 

1. EIS-based single-cell (S. cerevisiae) aging and lifespan determination, embryonic development monitoring and C. elegans worm sorting;

2. EIT-based worm locomotion and human regional lung function monitoring;

3. Machine-learning and neural-network algorithms for cell morphology analysis, animal behavior recognition and EIT image reconstruction.

Book Chapters

[3] 朱真^*, 译第18章封装技术在生物电子中的应用，《器件和系统封装技术与应用：原书第2版》，（美）拉奥R.图马拉（Rao R. Tummala）主编，李晨等译，北京：机械工业出版社，2021.03

[2] Z. Zhu^*, Y. Geng, and Y. Wang, Monitoring single S. cerevisiae cells with multifrequency electrical impedance spectroscopy in an electrode-integrated microfluidic device (Chapter 9). In: Marchisio M. A. (eds) Computational Methods in Synthetic Biology, Methods in Molecular Biology, vol 2189. Springer Nature, New York, NY, 2021. 

[1] Z. Zhu^*, O. Frey, and A. Hierlemann, Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo (Chapter 13). In: Singleton T. (eds) Schizosaccharomyces pombe: Methods and Protocols, Methods in Molecular Biology, vol 1721, Springer Nature, New York, NY, 2018.

Selected Publications

[33] Y. Wang, Z. Zhu*, K. Liu, Q. Xiao, Y. Geng, F. Xu, S. Ouyang, K. Zheng, Y. Fan, N. Jin, X. Zhao, M. A. Marchisio, D. Pan, and Q.-A. Huang, A high-throughput microfluidic diploid yeast long-term culturing (DYLC) chip capable of bud reorientation and concerted daughter dissection for replicative lifespan determination, Journal of Nanobiotechnology, 2022, 20: 171. (IF 10.435) https://doi.org/10.1186/s12951-022-01379-9 

[32] Y.-S. Tan, L. Wang, Y.-Y. Wang, Q.-E. He, Z.-H. Liu, Z. Zhu*, K. Song*, B.-Z. Li*, and Y.-J. Yuan, Protein acetylation regulates xylose metabolism during adaptation of Saccharomyces cerevisiae, Biotechnology for Biofuels, 2021, 14: 241. (IF 6.040)

[31] Z. Zhang, X. Huang, K. Liu, T. Lan, Z. Wang, and Z. Zhu*, Recent advances in electrical impedance sensing technology for single-cell analysis, Biosensors, 2021, 11: 470. (IF 5.519) https://doi.org/10.3390/bios11110470 

[30] 刘亚, 刘可, 黄庆安, 柏涛, 邱收, 张娜, 朱真^*, 基于视频深度学习的小鼠恐惧情绪识别与分析方法研究, 生命科学仪器, 2021, 19(4): 37-44. 

[29] 刘可, 王颖瀛, 耿杨烨, 肖秦, 朱真^*, 酿酒酵母单细胞形态参数精准提取算法的研究, 传感技术学报, 2021, 34(8): 1001-1006. 

[28] Z. Zhu*, Y. Geng, Y. Wang, K. Liu, Z. Yi, X. Zhao, S. Ouyang, K. Zheng, Y. Fan and Z. Wang*, Real-time monitoring of dissection events of single budding yeast in a microfluidic cell-culturing device integrated with electrical impedance biosensor, Frontiers in Bioengineering and Biotechnology, 2021, 9: 783428. (IF 5.890)  https://doi.org/10.3389/fbioe.2021.783428

[27] Y. Geng, Z. Zhu*, Z. Zhang, F. Xu, M. A. Marchisio, Z. Wang, D. Pan, X. Zhao, and Q.-A. Huang, Design and 3D modeling investigation of a microfluidic electrode array for electrical impedance measurement of single yeast cells, Electrophoresis, 2021, 42: 1996-2009. (IF 3.535) https://doi.org/10.1002/elps.202100028

[26] X.-P. Li, K.-Y. Qu, B. Zhou, F. Zhang, Y.-Y. Wang, O. D. Abodunrin, Z. Zhu, N.-P. Huang, Electrical stimulation of neonatal rat cardiomyocytes using conductive polydopamine-reduced graphene oxide-hybrid hydrogels for constructing cardiac microtissues, Colloids and Surfaces B: Biointerfaces, 2021, 205: 111844. (IF 5.268)

[25] Z. Zhu*, Y. Wang, R. Peng, P. Chen,Y. Geng, B. He, S. Ouyang, K. Zheng, Y. Fan, D. Pan, N. Jin, F. Rudolf, and A. Hierlemann, A microfluidic single-cell array for in situ laminar-flow-based comparative culturing of budding yeast cells, Talanta, 2021, 231: 122401. (IF 6.057) https://doi.org/10.1016/j.talanta.2021.122401

[24] F. Zhang, K.-Y. Qu, B. Zhou, Y. Luo, Z. Zhu, D.-J. Pan, C. Cui, Y. Zhu, M.-L. Chen, and N.-P. Huang*, Design and fabrication of an integrated heart-on-a-chip platform for construction of cardiac tissue from human iPSC-derived cardiomyocytes and in situ evaluation of physiological function, Biosensors and Bioelectronics, 2021, 179: 113080. (IF 10.618)

[23] X. Xu, Z. Zhu*, Y. Wang, Y. Geng, F. Xu*, M. A. Marchisio, Z. Wang, and D. Pan, Investigation of daughter cell dissection coincidence of single budding yeast cells immobilized in microfluidic traps, Analytical and Bioanalytical Chemistry, 2021, 413: 2181-2193. (IF 4.157) https://doi.org/10.1007/s00216-021-03186-x

[22] R. Cai, S. Guo, Y. Wu, S. Zhang, Y. Sun, S. Chen, P. Gao^∗, C. Zhu, J. Chen, Z. Zhu, L. Sun^∗, and F. Xu^∗, Lattice-resolution visualization of anisotropic sodiation degrees and revelation of sodium storage mechanisms in todorokite-type MnO₂ with in-situ TEM, Energy Storage Materials, 2021, 37: 345–353. (IF 17.789) 

[21] M. Dong, R. Fu, H. Min, Q. Zhang, H. Dong, Y. Pan, L. Sun^*, W. Wei, M. Qin, Z. Zhu^*, and F. Xu^*, In situ liquid cell transmission electron microscopy investigation on the dissolution-regrowth mechanism dominating the shape evolution of silver nanoplates, Crystal Growth & Design, 2021, 21: 1314−1322. (IF 4.076)

[20] 张钊, 耿杨烨, 朱真^*, 用于酵母细胞电阻抗检测的集成微电极阵列微流控芯片的有限元仿真研究, 电子器件, 2021, 44(2): 255-261. 

[19] 郭涛, 张钊, 杨浠, 潘德京, 彭年才, 朱真^*, 新型冠状病毒检测方法的研究, 名医, 2020, 19: 76-77.

[18] K. Huang, Y. Geng, X. Zhang, D. Chen, Z. Cai, M. Wang, Z. Zhu^*, and Z. Wang^*, A wide-band digital lock-in amplifier and its application in microfluidic impedance measurement, Sensors, 2019, 19: 3519. (IF 3.576)

[17] K. Jia, Z. Lu, F. Zhou, Z. Xiong, R. Zhang, Z. Liu, Y. Ma, L. He, C. Li, Z. Zhu, D. Pan, and Z. Lian, Multiple sgRNAs facilitate base editingmediated i-stop to induce complete and precise gene disruption, Protein & Cell, 2019. (IF 14.870)

[16] Y. Geng, Z. Zhu^*, Y. Wang, Y. Wang, S. Ouyang, K. Zheng, W. Ye, Y. Fan, Z. Wang, and D. Pan, Multiplexing microelectrodes for dielectrophoretic manipulation and electrical impedance measurement of single particles and cells in a microfluidic device, Electrophoresis, 2019, 40: 1436-1445. (IF 3.535) https://doi.org/10.1002/elps.201800433

[15] Z. Zhu^*, Y. Geng, Z. Yuan, S. Ren, M. Liu, Z. Meng, and D. Pan, A bubble-free microfluidic device for easy-to-operate immobilization, culturing and monitoring of zebrafish embryos, Micromachines, 2019, 10: 168. (IF 2.891)  https://doi.org/10.3390/mi10030168

[14] 耿杨烨, 潘任豪, 王颖瀛, 吴成均, 初慧杰, 朱真^*,用于流式细胞电穿孔的微流控芯片的研究, 电子器件, 2019, 42(3): 545-550. 

[13] Z. Zhu^*, W. Chen, B. Tian, Y. Luo, J. Lan, D. Wu, D. Chen, Z. Wang, and D. Pan, Using microfluidic impedance cytometry to measure C. elegans worms and identify their developmental stages, Sensors and Actuators B: Chemical, 2018, 275: 470-482. (IF 7.460) https://doi.org/10.1016/j.snb.2018.07.169

[12] Z. Zhu^*, X. Xu, L. Fang, D. Pan, and Q.-A. Huang, Investigation of geometry-dependent sensing characteristics of microfluidic electrical impedance spectroscopy through modeling and simulation, Sensors and Actuators B: Chemical, 2016, 235: 515-524. (IF 7.460) https://doi.org/10.1016/j.snb.2016.05.092

[11] Z. Zhu^*, P. Chen, K. Liu, and C. Escobedo, A versatile bonding method for PDMS and SU-8 and its application towards a multifunctional microfluidic device, Micromachines, 2016, 7: 230. (IF 2.891)  https://doi.org/10.3390/mi7120230

[10] Z. Zhu^*, O. Frey, N. Haandbæk, F. Franke, F. Rudolf, and A. Hierlemann, Time-lapse electrical impedance spectroscopy for monitoring the cell cycle of single immobilized S. pombe cells, Scientific Reports, 2015, 5: 17180. (IF 4.379)

[9] K. Liu, Z. Zhu^*, X. Wang, D. Gonçalves, B. Zhang, A. Hierlemann, and P. Hunziker^*, Microfluidics-based single-step preparation of injection-ready polymeric nanosystems for medical imaging and drug delivery, Nanoscale, 2015, 7: 16983-16993. (IF 7.790)

[8] Z. Zhu^*, O. Frey, F. Franke, N. Haandbæk, and A. Hierlemann, Real-time monitoring of immobilized single yeast cells through multi-frequency electrical impedance spectroscopy, Analytical and Bioanalytical Chemistry, 2014, 406: 7015-7025. (IF 4.157)

[7] Z. Zhu^*, O. Frey, D. S. Ottoz, F. Rudolf, and A. Hierlemann, Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells, Lab on a Chip, 2012, 12: 906-915. (IF 6.799)

[6] Z. Zhou, Q.-A. Huang^*, Z. Zhu, and W. Li, An efficient simulation system for inclined UV lithography processes of thick SU-8 photoresists, IEEE Transactions on Semiconductor Manufacturing, 2011, 24(2): 294-303. (IF 2.874)

[5] Z. Zhu, Z. Zhou, Q.-A. Huang^*, and W. Li, Modeling, simulation and experimental verification of inclined UV lithography for SU-8 negative thick photoresists, Journal of Micromechanics and Microengineering, 2008, 18: 125017-125027. (IF 1.881)

[4] Z. Zhou, Q.-A. Huang^*, W. Li, M. Feng, W. Lu, and Z. Zhu, Improvement of the 2D dynamic CA method for photoresist etching simulation and its application to deep UV lithography simulations of SU-8 photoresists, Journal of Micromechanics and Microengineering, 2007, 17: 2538-2547. (IF 1.881)

[3] 朱真^*, 黄庆安, 李伟华, 周再发, 冯明, SU-8胶曝光衍射效应的模拟及丙三醇补偿方法, 半导体学报, 2007, 28(12): 2011-2017.

[2] 冯明, 黄庆安, 李伟华, 周再发, 朱真, SU-8胶深紫外光刻模拟, 半导体学报, 2007, 28(9): 1465-1470.

[1] 冯明, 黄庆安^*, 李伟华, 周再发, 朱真, SU-8胶在深紫外光源下的光强分布模拟, 传感技术学报, 2006, 19(5): 1470-1476.

Patents

[12] 朱真，徐星宇，王昊曦，杨剑坤，一种用于线虫运动行为和生理特征监测的微流控芯片，2022.03.29，中国，ZL202010474150.1